Products Category for Conventional (Standard) PCR with DNA
Product Code :
18500
Description :
AddStart Taq DNA Polymerase has bounded a unique monoclonal anti-Taq antibody that is specific to normal Taq DNA Polymerase. This unique feature of hot-start Taq DNA Polymerase product allows for minimal primer dimers and nonspecific amplification reactions and maximizing the amplification efficiency of the target gene region. AddStart Taq DNA Polymerase possesses 5'→3' exonuclease activity and very weak 3'→5' exonuclease activity. Additionally, since an A-tail is formed at the 3' end of the amplified DNA, a T-vector can be used for cloning using the amplification product. When utilizing AddStart Taq DNA Polymerase for PCR, 5 kb or less of amplification products can be obtained under standard PCR conditions. It is also suitable for multiplex PCR that can amplify 5 or fewer targets simultaneously. The 10x reaction buffer contains a range of components to maximize the activity of the hot-start Taq DNA Polymerase, along with 20mM MgCl2.
Product Code :
36101, 36101U, 36101SS, 36101USS
Description :
AddStart Taq DNA Master (2x Conc.) is a pre-mixed product of essential components for PCR reactions, including AddStart Taq DNA Polymerase, Reaction Buffer (+Mg), dNTP mixture, gel loading dye (Xylene Cyanol FF), and enzyme stabilizers to maximize customers' convenience. AddStart Taq DNA Master (2x Conc.) ensures the same performance as the manual product and can produce amplification products of up to 5 kb under standard PCR conditions. Additionally, since an A-tail is formed at the 3' end of the amplified DNA, the T-vector can be used for cloning using the amplification product. This product is supplied in two forms of 1.0 mL per screw cap tube (Cat. No. 36101) and 10.0 μl per 8-strip tube (Cat. No. 36101SS). Furthermore, to prevent carryover contamination from amplification products, AddStart Taq DNA Master with UDG includes UracilDNA Glycosylase (UDG) and is available in two forms of 1.0 mL per screw cap tube (Cat. No. 36101U) and 10.0 μl per 8-strip tube (Cat. No. 36101USS).
Product Name | Product Code | Provided with |
---|---|---|
AddStart Taq DNA Polymerase | 18500 | AddStart Taq DNA Polymerase (5 U/μl), 100 μl 10x Reaction Buffer 1.0 mL 10mM dNTP Mix. (2.5 mM each) 1.0 mL |
AddStart Taq DNA Master without UDG (2x conc.) | 36101 (tube type) | 1.0 mL x 5 tubes |
36101SS (8-strip type) | 10.0 μl / tube x 96 tests | |
AddStart Taq DNA Master with UDG (2x conc.) | 36101U (tube type) | 1.0 mL x 5 tubes |
36101USS (8-strip type) | 10.0 μl / tube x 96 tests |