DNA/RNA Amplification

DNA/RNA Amplification

Standard PCR

Products Category for Conventional (Standard) PCR with DNA

01. Add Taq DNA Polymerase

Product Code :

17500

Description :

Add Taq DNA Polymerase is a recombinant protein derived from the Thermus aquaticus DNA Polymerase I gene, expressed and purified from recombinant Escherichia coli. Taq DNA Polymerase is the most commonly used in PCR method. It possesses 5'→3' exonuclease activity as a characteristic of Taq DNA Polymerase and very weak 3'→5' exonuclease activity. In addition, since an A-tail is formed at the 3' end of the amplified DNA, a T-vector can be used for cloning using the amplification product.
When utilizing Add Taq DNA Polymerase for PCR, 3 kb or less of amplification products can be obtained under standard PCR conditions. The 10x reaction buffer contains a range of components to maximize the activity of Taq DNA Polymerase, along with 20 mM MgCl2.

02. Add Taq DNA Master, 2x conc.

Product Code :

35001, 35001SS

Description :

Add Taq DNA Master (2x Conc.) is a pre-mixed product of essential components for PCR reactions, including Add Taq DNA Polymerase, Reaction Buffer (+Mg), dNTP mixture, gel loading dye (Xylene Cyanol FF), and enzyme stabilizers to maximize customers' convenience. Add Taq DNA Master (2x Conc.) ensures the same performance as the manual product and can produce amplification products of up to 3 kb under standard PCR conditions. Additionally, since an A-tail is formed at the 3' end of the amplified DNA, the T-vector can be used for cloning using the amplification product. This product is supplied in two forms of 1.0 mL per screw cap tube (Cat. No. 35001) and 10.0 μl per 8-strip tube (Cat. No. 35001SS).

03. Performance Data
04. Order Information
Product Name Product Code Provided with
Add Taq DNA Polymerase 17500 Add Taq DNA Polymerase (5 U/μl), 100 μl
10x Reaction Buffer 1.0 mL
10mM dNTP Mix. (2.5 mM each) 1.0 mL
Add Taq DNA Master (2x Conc.) 35001 (tube type) 1.0 mL x 5 tubes
35001SS (8-strip type) 10.0 μl / tube x 96 tests
* For bulk order and customized specifications, please inquire separately.