DNA/RNA Amplification

DNA/RNA Amplification

Real-time PCR

Products Category for probe-based qPCR assay and RT-qPCR assay

01. AddFast qPCR Master (without or with UDG), 2x conc.

Product Code :

72401TN, 72401TNU

Description :

AddFast qPCR Master is a product exclusive for fast real-time PCR using a fluorescence probe (TaqMan Probe). This product contains hot-start Taq DNA Polymerase, dNTP mixture, and other components necessary for PCR. Particularly, the amplification speed and efficiency are outstanding by incorporating additives for Fast qPCR and buffer components optimized for real-time PCR. And results can be obtained within approximately 40-50 minutes. As the AddFast qPCR Master with UDG includes Uracil-DNA Glycosylase (UDG) to prevent carryover contamination from amplification products, it is suitable for molecular diagnostic applications.

02. AddFast RT-qPCR Master (without or with UDG), 2x conc.

Product Code :

74201TN, 74201TNU

Description :

The AddFast RT-qPCR Master is an exclusive product for fast real-time PCR that utilizes a fluorescence probe (TaqMan Probe). This product contains various enzymes and components necessary for one-step RT-qPCR, including hot-start Taq DNA Polymerase, Reverse Transcriptase (RNase H-), RNase inhibitor, and other PCR-related components to maximize user convenience. In particular, amplification speed and efficiency are outstanding by incorporating additives for Fast RT-qPCR and buffer components optimized for real-time PCR. And results can be obtained within approximately 50 minutes to 1 hour. The AddFast RT-qPCR Master with UDG includes heat-labile Uracil-DNA Glycosylase (UDG) to prevent carryover contamination from amplification products and lost activity at 25℃. Those allow removing contaminants without affecting cDNA synthesis and make it suitable for molecular diagnostic applications.

03. Performance Data
04. Order Information
Product Name Product Code Provided with
AddFast qPCR Master (2x conc.) 72401TN 1.0 mL x 1 tube
AddFast qPCR Master with UDG (2x conc.) 72401TNU 1.0 mL x 1 tube
AddFast RT-qPCR Master (2x conc.) 74201TN 1.0 mL x 1 tube
AddFast RT-qPCR Master with UDG (2x conc.) 74201TNU 1.0 mL x 1 tube
* For bulk order and customized specifications, please inquire separately.